Metabolic substrates alter attachment and differentiated functions of proximal tubule cell culture.
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چکیده
منابع مشابه
The Proximal Tubule Cell in Experimental Hydronephrosis
The purpose of this report is to record several observations made in a study of hydronephrosis produced in rats by unilateral ligation of the ureter. I t has been known from the work of Wilmer (1) and Wachstein (2, 3) that the staining of the proximal tubule brush border for alkaline phosphatase activity is diminished in experimental hydronephrosis. However, the speed with which this decrease o...
متن کاملEffect of metabolic acidosis on neonatal proximal tubule acidification.
The serum bicarbonate in neonates is lower than adults due in large part to a lower rate of proximal tubule acidification. It is unclear if the neonatal proximal tubule is functioning at maximal capacity or if the proximal tubule can respond to metabolic acidosis as has been described in adult proximal tubules. We find that neonatal mouse brush-border membranes have a lower Na(+)/H(+) exchanger...
متن کاملMaladaptive proximal tubule repair: cell cycle arrest.
Acute kidney injury (AKI) leads to worsening of chronic kidney disease (CKD), and CKD predisposes to the clinical entity of AKI. The tubules of the kidney play a central role in the fibrotic response, which ultimately leads to progressive kidney disease. The cellular mechanisms responsible for the epidemiological association between AKI and CKD are complex. In order to unravel characteristics o...
متن کاملCharacterization of a primary cell culture model of the avian renal proximal tubule.
Methods have been developed for producing functional, transporting monolayers of avian proximal tubule (PT) cells. A highly homogenous fraction of PT fragments was prepared by enzymatic digestion (collagenase + Dispase) of chick (3- to 5-day-old) kidneys, followed by Percoll gradient centrifugation. The PT fraction was enriched in glucose-6-phosphatase, a proximal enzyme marker, and reduced in ...
متن کاملMaladaptive Proximal Tubule Repair: Cell Cycle Arrest
Acute kidney injury (AKI) leads to worsening of chronic kidney disease (CKD), and CKD predisposes to the clinical entity of AKI. The tubules of the kidney play a central role in the fibrotic response, which ultimately leads to progressive kidney disease. The cellular mechanisms responsible for the epidemiological association between AKI and CKD are complex. In order to unravel characteristics o...
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ژورنال
عنوان ژورنال: Journal of the American Society of Nephrology
سال: 1994
ISSN: 1046-6673,1533-3450
DOI: 10.1681/asn.v4111908